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Mixture deconvolution utilizes EuroForMix (EFM).
There are several options/settings to run EFM mixture deconvolution:
The type of mixture deconvolution analysis to perform (one or both can be selected at once):
- Unconditioned analysis
- Conditioned analysis
Allele Frequency Data: The user must choose which allele frequency data to use: 1000Genomes Phase 3 data, gnomAD v4 data, or upload a custom file. See above for more details about the format for uploading a custom AF file.
References to Condition on: IF running a conditioned analysis, once the reference folder has been uploaded, this dropdown menu will auto-populate containing the reference sample IDs. The user must select which references to condition on. As of now, MixDeR can only condition on a single reference sample. However, multiple references can be selected for conditioning; the conditioned analyses will be run separately.
Number of SNP Bins: The number of SNP sets for each sample. The default is 10.
Static Analytical Threshold: The minimum number of reads required for a SNP to be included (default = 10).
Dynamic Analytical Threshold: The percent of total SNP reads required for a SNP to. be included (default = 0.015 or 1.5%). (A quick note on the ATs: both the static and dynamic ATs are applied and the one producing the higher AT will be used in the EFM software).
Output Folder Name: The name of the folder created within the folder containing the original SNP files to store the generated output for the entire workflow.
Minimum Number of SNPs: The minimum number of SNPs